The complete plastid Wallets genome of Holcoglossum tsii was determined and analyzed in this work.The plastome was 146,897 bp in length with 83,366 bp of the large single-copy (LSC) region, 11,957 bp of the small single-copy (SSC) region, and 25,787 bp of the invert repeats (IR) regions.The genome contained 127 genes, 74 protein-coding genes, 38 tRNA genes, and 8 rRNA genes.Phylogenetic analysis suggested Creatine H.tsii is sister to H.
rupestre.